Spectrophotometry is the quantifiable study of electromagnetic spectra. Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color (or more specifically the wavelength) of light. A NIR spectrophotometer is commonly used to measure light absorption and diffuse or specular reflectance. However, spectrophotometers are not only used in physics; it can also be used in other fields such as biochemistry, chemistry, and molecular biology. A NIR spectrophotometer works by first inserting the sample into the instrument. You must have a light source that shines into the monochromator. A particular wavelength that is beamed at the sample is selected, and in turn, the sample absorbs the light. There are two common kinds of spectrophotometers: the UV VIS NIR spectrophotometer, and the IR spectrophotometer. The former stands for ultraviolet visible near infrared spectrophotometer. It is so called because the light used is in the visible and adjacent ranges, such as the near ultraviolet and near infrared ranges. The second type of NIR spectrophotometer is the IR or infrared spectrophotometer. It is designed for the main infrared region and is less commonly used compared to the UV VIS NIR spectrophotometer. The use of spectrophotometers is not limited to studies in physics. They are also commonly used in other scientific fields such as chemistry, biochemistry, and molecular biology. When planning to purchase general purpose spectrophotometers for your laboratory settings, make sure to utilize the services of competent distributors in the industry.
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